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Event Type:  Seminar (This is an NIH Science event)
Title:  Biowulf Seminar Series: Cryo-EM studies of glutamate receptors and nucleosomes
Description:  Abstract: Electron microscopy (EM) has been traditionally used to calculate three-dimensional reconstructions that were interpreted using high-resolution structures determined by X-ray crystallography and NMR to provide biological insights into the function of large macromolecular assemblies. However, recent technological and methodological developments in cryo-EM have now made it possible to routinely study biological macromolecules of lower molecular weight at near-atomic resolutions, thus opening up an interesting area of determining three dimensional structures of novel complexes. Here, I will discuss the following two examples to highlight the role of single particle cryo-EM in advancing the structural biology: 1. Ionotropic glutamate receptors (iGluRs) – here we used single particle cryo-EM in conjunction with high resolution structures determined by X-ray crystallography to reveal key conformational changes involved in receptor gating [references 1 and 2]. 2. Structure of the centromeric nucleosome in complex with the kinetochore protein CENP-N – to illustrate an example where we utilized the cryo-EM density map to carry out ab initio modeling of the CENP-N component without any prior knowledge of its three-dimensional structure [reference 3]. With these and ongoing advances in cryo-EM, it is now feasible to study other previously intractable biological complexes.

[1] Structural mechanism of glutamate receptor activation and desensitization (2014). Nature 514:328-334

[2] Structural basis of kainate subtype glutamate receptor desensitization (2016). Nature 537: 567-571

[3] Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N (2018). Science 359: 339-343

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Series Name:  Biowulf Seminar Series
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Wednesday, May 09, 2018   11:00am - 12:00pm Add To Outlook Calendar     Add To iCal Calendar     Add To Entourage Calendar
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Name:   Sagar Chittori
Title:   Research Fellow
Organization:   LCB, NCI

Organization(s):  NIH Biowulf Core Computing Facility

Location:  On the main NIH Campus
Building:  Building 50
Room:  1227 (next to the coffeeshop)

Name:   Susan Chacko
Phone:   301-435-2982
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