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EVENT DESCRIPTION  
 
Event Type:  Seminar (This is an NIH Science event)
 
Title:  Biowulf Seminar Series: Cryo-EM studies of glutamate receptors and nucleosomes
 
Description:  Abstract: Electron microscopy (EM) has been traditionally used to calculate three-dimensional reconstructions that were interpreted using high-resolution structures determined by X-ray crystallography and NMR to provide biological insights into the function of large macromolecular assemblies. However, recent technological and methodological developments in cryo-EM have now made it possible to routinely study biological macromolecules of lower molecular weight at near-atomic resolutions, thus opening up an interesting area of determining three dimensional structures of novel complexes. Here, I will discuss the following two examples to highlight the role of single particle cryo-EM in advancing the structural biology: 1. Ionotropic glutamate receptors (iGluRs) – here we used single particle cryo-EM in conjunction with high resolution structures determined by X-ray crystallography to reveal key conformational changes involved in receptor gating [references 1 and 2]. 2. Structure of the centromeric nucleosome in complex with the kinetochore protein CENP-N – to illustrate an example where we utilized the cryo-EM density map to carry out ab initio modeling of the CENP-N component without any prior knowledge of its three-dimensional structure [reference 3]. With these and ongoing advances in cryo-EM, it is now feasible to study other previously intractable biological complexes.



[1] Structural mechanism of glutamate receptor activation and desensitization (2014). Nature 514:328-334

[2] Structural basis of kainate subtype glutamate receptor desensitization (2016). Nature 537: 567-571

[3] Structural mechanisms of centromeric nucleosome recognition by the kinetochore protein CENP-N (2018). Science 359: 339-343



Lab webpage: https://electron.nci.nih.gov
 
Series Name:  Biowulf Seminar Series
 
Event URL:  https://hpc.nih.gov/training/
 
Videocast:  Event will be videocast LIVE on the Web
 
Videocast URL:  http://videocast.nih.gov
  Event will be available in the videocast ARCHIVE
 
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EVENT DATE/TIME  
 
Date/Time: 
Wednesday, May 09, 2018   11:00am - 12:00pm Add To Outlook Calendar     Add To iCal Calendar     Add To Entourage Calendar
 
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EVENT SPEAKER(S)  
 
Name:   Sagar Chittori
Title:   Research Fellow
Organization:   LCB, NCI
 

EVENT SPONSOR(S)  
 
Organization(s):  NIH Biowulf Core Computing Facility
 

EVENT LOCATION  
 
Location:  On the main NIH Campus
Building:  Building 50
Room:  1227 (next to the coffeeshop)
 
 

EVENT CONTACT(S)  
 
Name:   Susan Chacko
E-mail:   susanc@helix.nih.gov
Phone:   301-435-2982
 
 
 
 
 
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