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Event Type:  Seminar (This is an NIH Science event)
Annual Lecture:  Other
Title:  Super Resolution as a Tool to Study a Potential Role for DNA Replication in Establishing Distinct Epigenomes
Description:  The primary function of DNA replication is to duplicate the genome. However, the process of DNA replication must also duplicate epigenetic information (Alabert and Groth, 2012; Yadav and Whitehouse, 2016). Epigenetic mechanisms play a key role in altering chromatin structure and gene expression patterns, and in specifying and maintaining stem cell identity throughout cell division (Allis and Jenuwein, 2016; Atlasi and Stunnenberg, 2017; Bonasio et al., 2010; Probst et al., 2009; Vassilev and DePamphilis, 2017). Many types of stem cells have the ability to asymmetrically divide to give rise to one daughter cell capable of self-renewal and another daughter capable of differentiating.
Using a dual-color labeling system, we demonstrated that in the Drosophila germline H3 is inherited asymmetrically whereas the H3 variant H3.3 is inherited symmetrically (Tran et al., 2012). As H3 is incorporated during S-phase whereas H3.3 is incorporated in a replication-independent manner (Ahmad and Henikoff, 2002b), we hypothesize that old and new H3 are differentially incorporated into distinct sister chromatids during DNA replication.
Because the average diameter of replication forks in eukaryotic cells (~150 - 400 nm) (Cseresnyes et al., 2009; Leonhardt et al., 2000; McKnight and Miller, 1977) is at or below the diffraction-limited resolution of conventional fluorescence light microscopy (250 - 300 nm), we used single molecule localization based super-resolution (SR) imaging to examine the spatial distribution of old and new H3 and H3.3 in interphase GSCs (Betzig et al., 2006). Interestingly, we were able to observe that these two histone species show significantly different co-localization patterns throughout interphase.
We have developed a method using STED SR microscopy in tandem with the chromatin fiber technique (Ahmad and Henikoff, 2002a; Blower et al., 2002; Cohen et al., 2009; Hell and Wichmann, 1994; Sahl et al., 2017) to observe asymmetries in DNA replication as
Series Name:  Light Microscopy Interest Group Seminar
Videocast:  Event will not be videocast
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Tuesday, January 16, 2018   11:00am - 12:00pm Add To Outlook Calendar     Add To iCal Calendar     Add To Entourage Calendar
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Name:   Matthew Wooten
Title:   Dr
Organization:   Johns Hopkins U Sch Med
City/Province:   Baltimore
State:   Maryland
Country:   USA

Organization(s):  [NIH] Light Microscopy Interest Group

Location:  On the main NIH Campus
Building:  37
Room:  4107/4041
Street Address:  37 Convent Drive
City:  Behesda
State:  Maryland
Zip Code:  20892

Name:   Tatiana Karpova
Phone:   240-760-6637
Fax:   240-541-4450
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