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Event Type:  Seminar (This is an NIH Science event)
Annual Lecture:  Other
Title:  Probing the physical properties of the microenvironment in vivo
Description:  Tissue is composed of heterogeneous biological components that modulate physical properties within the microenvironment. Transformation of the physico-chemical properties of the stromal microenvironment such as changes in the extracellular matrix (ECM) has been shown to be associated with cancer progression. Cells respond to both chemical and physical cues of the microenvironment. In tissue, chemical and mechanical cues are both modulated by changes in ligand density and localized tissue architecture. Hence, decoupling chemical cues from those due to the physical changes is non-trivial. What is needed is the ability to resolve and quantitate minute forces that cells sense in the local environment (on the order of microns) within thick tissue (in mm). To achieve this, we employ a method to quantitate absolute tissue mechanics using in vitro and in vivo models. We performed Active Microrheology by optical trapping in vivo, using in situ calibration to accurately apply and measure forces. With micrometer resolution at broadband frequencies and depths approaching 0.5 mm, we probed differential stresses and strains on force, time and length scales relevant to cellular processes in living zebrafish. We determined that proxy calibration methods overestimate complex moduli by as much as ~20 fold. While ECM hydrogels displayed rheological properties predicted for polymer networks, new models may be needed to describe the behavior of tissues observed. Finally, we validated our in vitro findings in an in vivo model using zebrafish as our model for metastasis. We believe that this platform can be used in elucidating the basic mechanisms that govern the role of material properties in mechanobiology.
1. Staunton J.R., Blehm, B.H., Devine, A., Tanner, K. In situ calibration of position detection in an optical trap for active microrheology in viscous materials, (Optics Express, In press 2017)
2. Staunton J.R, Vieira, W., Fung Leung, K., Lake R Devine, A, Tanner, K
Series Name:  Light MIcroscopy Interest Group Seminar
Videocast:  Event will not be videocast
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Tuesday, November 14, 2017   11:00am - 12:00pm Add To Outlook Calendar     Add To iCal Calendar     Add To Entourage Calendar
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Name:   Kandice Tanner
Title:   Dr
Organization:   NCI
City/Province:   Bethesda
State:   Maryland
Country:   USA

Organization(s):  [NIH] Light Microscopy Interest Group

Location:  On the main NIH Campus
Building:  37
Room:  4107/4041
Street Address:  37 Convent Drive
City:  Bethesda
State:  Maryland
Zip Code:  20892

Name:   Tatiana Karpova
Phone:   240-760-6637
Fax:   240-541-4450
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