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Event Type:  Seminar (This is an NIH Science event)
Annual Lecture:  Other
Title:  Oh The Places LAT Goes!
Description:  When a T cell engages cells bearing foreign antigens and becomes activated, an initial event is the formation of structures called microclusters at the contact surface between the cells. Little is understood about how those microclusters form. Some have hypothesized that membrane proteins on the surface of the T cell migrate to the area of contact; others have hypothesized that proteins from cytoplasmic vesicles in the T cell are recruited to form the microclusters.¬¬ To understand the key events that lead to microcluster formation, we imaged an essential transmembrane signaling molecule LAT using lattice light sheet microscopy (LLSM). We observed a kinetic lag between LAT microcluster formation and vesicular pool recruitment to the synapse. We also looked at the activated T cell surface with higher spatial and temporal resolution using Total Internal Reflection Fluorescence Simulated Interference Microscopy (TIRF-SIM). We captured directed vesicle movement between microclusters where they exhibit decreased motility. We propose a model in which cell surface LAT is rapidly recruited and phosphorylated at sites of T cell activation, and the vesicular pool is recruited at later time points to replenish LAT at activation sites.
Key references:
The linker for activation of T cells (LAT) signaling hub: from signaling complexes to microclusters. Balagopalan L, Kortum RL, Coussens NP, Barr VA, Samelson LE. J Biol Chem. 2015 Oct 30;290(44):26422-9.
Cell surface linker for activation of T cells is recruited to microclusters and is active in signaling. Balagopalan L, Barr VA, Kortum RL, Park AK, Samelson LE., J Immunol. 2013 Apr 15;190(8):3849-53.
Series Name:  Light Microscopy Interest Group (LMIG) Seminar
Videocast:  Event will not be videocast
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Tuesday, March 21, 2017   11:00am - 12:00pm Add To Outlook Calendar     Add To iCal Calendar     Add To Entourage Calendar
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Name:   Lakshmi Balagopalan
Title:   Dr
Organization:   NIH
City/Province:   Bethesda
State:   Maryland
Country:   USA

Organization(s):  [NIH] Light Microscopy Interest Group

Location:  On the main NIH Campus
Building:  37
Room:  4107/4041
Street Address:  37 Convent Drive
City:  Bethesda
State:  Maryland
Zip Code:  20892

Name:   Tatiana Karpova
Phone:   240-7606-637
Fax:   240-541-4450
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